Finding markers to predict if a cancer patient will respond to treatment is a top priority. Using single-cell RNA sequencing, scientists have found specific genes and cellular states linked to how well a patient responds to treatment, but these markers only work for specific types of cancer. The Yizhak lab is analyzing ~1 million immune cells from cancer patients who received immunotherapy, to try to identify the best cellular groups and genes for accurate predictions and to determine the most effective biomarkers for treating patients using immunotherapy.